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NRF2 activation attenuated IL-6-induced MCP-1 production.

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posted on 2017-09-08, 17:54 authored by Timothy K. Eitas, Wesley Stepp, Lucas Sjeklocha, Clayton Long, Caitlin Riley, James Callahan, Yolanda Sanchez, Peter Gough, Laquanda Knowlin, David van Duin, Shiara Ortiz-Pujols, Samuel Jones, Robert Maile, Zhi Hong, Scott Berger, Bruce Cairns

(A) ELISA analysis for MCP-1 in healthy donor monocytes after treatment with CDDO-Me(bardoxolone methyl) (50nM) at indicated Hours Post-IL-6 Stimulation (HPS). Results are representative of 4 independent donors. (B) Bar graph depicting the percentage of CDDO-Me(bardoxolone methyl)-altered MCP-1 production at 16 HPS. Relative values represent the combination of 4 independent sets of healthy donor monocytes. (C) Immunoblot analysis demonstrating NQO1 expression after treatment with CDDO-Me(bardoxolone methyl) (50nM) at 16 HPA. Results are representative of 2 independent donors. (D) ELISA analysis for MCP-1 in healthy donor monocytes after treatment with Compound 7 (1 μM) at 16 HPS. Results are representative of 2 independent donors. Error bars represent SEM. * = p < .05. ** = p < .01. *** = p < .001. **** = p < .0001.

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