figshareBrowse
Fig 5.tif (391.32 kB)
Download file

NRF2 activation attenuated IL-6-induced MCP-1 production.

Download (391.32 kB)
figure
posted on 2017-09-08, 17:54 authored by Timothy K. Eitas, Wesley Stepp, Lucas Sjeklocha, Clayton Long, Caitlin Riley, James Callahan, Yolanda Sanchez, Peter Gough, Laquanda Knowlin, David van Duin, Shiara Ortiz-Pujols, Samuel Jones, Robert Maile, Zhi Hong, Scott Berger, Bruce Cairns

(A) ELISA analysis for MCP-1 in healthy donor monocytes after treatment with CDDO-Me(bardoxolone methyl) (50nM) at indicated Hours Post-IL-6 Stimulation (HPS). Results are representative of 4 independent donors. (B) Bar graph depicting the percentage of CDDO-Me(bardoxolone methyl)-altered MCP-1 production at 16 HPS. Relative values represent the combination of 4 independent sets of healthy donor monocytes. (C) Immunoblot analysis demonstrating NQO1 expression after treatment with CDDO-Me(bardoxolone methyl) (50nM) at 16 HPA. Results are representative of 2 independent donors. (D) ELISA analysis for MCP-1 in healthy donor monocytes after treatment with Compound 7 (1 μM) at 16 HPS. Results are representative of 2 independent donors. Error bars represent SEM. * = p < .05. ** = p < .01. *** = p < .001. **** = p < .0001.

History

Usage metrics

Read the peer-reviewed publication

Differential regulation of innate immune cytokine production through pharmacological activation of Nuclear Factor-Erythroid-2-Related Factor 2 (NRF2) in burn patient immune cells and monocytes
PLOS ONEPLOS ONE

Categories

Keywords

CCLIL -6. Pharmacological NRF 2 activationTBSAmonocytes Burn patientspatient Peripheral Blood Mononuclear CellsMonocyte Chemoattractant ProteinIL -6IL -10 secretionMOABurn Surface AreaPBMCNRF 2 agonistscytokines Interleukin -6Similar immunomodulatory effects-10 transcript levelsNRF 2 pathwayNuclear Factor-Erythroid -2-Related Factor 2HPANRF 2 activationMCP

Licence

CC BY 4.0

Exports