N99 acts as a specificity determinant.
figure
posted on 2024-02-13, 18:27 authored by Jia Wei Siau, Asim Azhar Siddiqui, Sze Yi Lau, Srinivasaraghavan Kannan, Sabrina Peter, Yingying Zeng, Chandra Verma, Peter Droge, John F. GhadessyThe indicated integrases were made using in vitro translation and incubated with attNanno1 X attNanno1L plasmid substrate. Scoring of recombination post incubation was carried out by end-point PCR using the primer 3 + 4 pair. Recombination measured by these primers yields a 151 bp band (arrowed).
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targeted genomic insertionefficiently targeted usingnatural target sitesendogenous target motifscopy target motifphaeodactylum tricornutum nannochloropsis oceanica large dna fragmentsdna editing toolboxdiv >< ptarget genomesvitro dna sequencestherefore requiredspecificity towardsrecombinase enzymesparticular applicationhuman genomehuman cellsgreen biomanufacturingfold switchextremely efficientextensive engineeringengineered integrasedirected evolutioncarbon sequestrationbroaden applicability
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