N380D substitution does not impact HA thermal stability.

Additional accelerated thermostability assays were performed with A/SLOV15 N380D (dark blue) and A/SLOV15 S12 (light purple). A/SLOV15 V1 (light blue), A/SLOV15 S13 (dark purple), A/NC99 (black) and A/CA09 (red) LAIV viruses were included for reference. A/SLOV15 N380D, A/SLOV15 S12, A/SLOV15 V1, A/SLOV15 S13, A/NC99 and A/CA09 viruses were held at temperatures ranging from 47.5°C—65°C for 20 minutes. After incubation, all samples underwent haemagglutination assays using 0.5% chicken red blood cells and log₂ titres were recorded.

(TIF)