Myelination of brains exposed to hyperoxia and steroids.
(A) Representative images of cingulate white matter stained with myelin basic protein (MBP; green) and with 4ʹ,6-diamidino-2-phenylindole (DAPI; blue) (scale bar, 200 μm), and (B) the average light intensity of MBP per high-power field (HPF) in cingulate white matter was measured at P14 at P14 (n = 6, 5, 6, 6 and 5 in NCV, HCV, HBV, HVD and HBD, respectively). Data are given as mean ± SEM. * P < 0.05 vs. NCV; # P < 0.05 vs. HCV; $ P < 0.05 vs. HBV. NCV, normoxia control with antenatal vehicle and postnatal vehicle; HCV, hyperoxia control with antenatal vehicle and postnatal vehicle; HBV, hyperoxia with antenatal betamethasone and postnatal vehicle; HVD, hyperoxia with antenatal vehicle and postnatal dexamethasone; and HBD, hyperoxia with antenatal betamethasone and postnatal dexamethasone.