Multiplexed siRNAs retain comparable efficacy to that exhibited by their individual counterparts
figureposted on 31.12.2011, 07:18 by Scott E. Martin, Tamara L. Jones, Cheryl L. Thomas, Philip L. Lorenzi, Dac A. Nguyen, Timothy Runfola, Michele Gunsior, John N. Weinstein, Paul K. Goldsmith, Eric Lader, Konrad Huppi, Natasha J. Caplen
Taken from "Multiplexing siRNAs to compress RNAi-based screen size in human cells"
Nucleic Acids Research 2007;35(8):e57-e57.
Published online 28 Mar 2007
© 2007 The Author(s)() Two multiplexes consisting of six siRNAs corresponding to three targets (two siRNAs per target) were evaluated for their ability to reduce cognate mRNA levels as compared to the activity of respective individual siRNAs in MDA-MB-231 cells. Total siRNA concentrations were held constant for all experiments such that individual siRNAs (siRNA-1 and siRNA-2) were tested at 60 nM and multiplexed siRNAs were tested at an individual siRNA concentration of 10 nM (60 nM total). Target-specific mRNA levels were compared to those found in cells transfected with negative-control siRNA. () Same as in () except that the multiplex consisted of twelve siRNAs corresponding to six targets. In this case, the total siRNA concentration was held constant at 120 nM again using 10 nM of each individual component within the multiplex. () Same as in () except that the experiments were conducted in MCF10A cells. Similarly, multiplexes retained activity when using gene targets other than those described here or when conducting studies in additional cell lines (data not shown).