pone.0256615.g003.tif (2.22 MB)
Multiplex immunofluorescent staining of ovarian tissues in Cohort 2.
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posted on 2021-11-23, 18:25 authored by Daryoush Saeed-Vafa, Douglas C. Marchion, Susan M. McCarthy, Ardeshir Hakam, Alexis Lopez, Robert M. Wenham, Sachin M. Apte, Dung-Tsa Chen, Anthony M. Magliocco, Johnathan M. Lancaster, Brett M. Reid, Jennifer B. PermuthRepresentative staining for high-grade ovarian serous adenocarcinoma (top) and benign ovarian tissue (bottom) are provided. Compartmental regions were identified based on DAPI, PanCK, and DAPI staining. Tumor (desmoplastic) stroma regions comprised stromal cells (Vimentin+) with a nucleus (DAPI+), and not epithelial (panCK-).
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utilizing digital pathologyusing multiplex immunofluorescenceoverall survival correlatedexplore therapeutic implicationsbenign ovarian tumorsbenign ovarian tissueovarian serous carcinomas>< sup ><neoplastic stroma using1 downregulation compareddiv >< pquantify stromal caveolinovca stroma compared1 expression (<epithelial expression (<stromal caveolinserous ovca1 expression>specific expressionexpression levelovca ).tumor epitheliumstaining assaysignificantly downregulatedseveral cancersr poor outcomesneither responseclinicopathological parametersclinical significanceassociated fibroblast98 ).2
