Mtb infection induced alternate polyadenylation in THP-1 macrophages.
(A) Number of transcripts in the human genome known to have 1, 2 to 5, 6 to 10 or more than 10 reported 3’UTRs is shown here as obtained from NCBI database. (B) Plot comparing the length of 3’UTR and corresponding FPKMs across the uninfected, H37Ra infected or H37Rv infected macrophages is shown here for 36 hour time point. (C) Percent distal poly-A uses index (PDUI) for transcripts and corresponding FPKM values are plotted here. Transcripts falling below the PDUI threshold score of ±0.2 and corresponding expression level below 2 are shown in grey. Increased expression is highlighted by red and decreased expression by blue.