Modulation of EMT and the formation of pseudopodia in the colon cancer cells following PRR14 depletion.

(A) Morphologic changes in mesenchymal HCT116 and RKO cells after transfection with siNC and siPRR14. Scale bar, 50 μm. (B) Western blot was used to detect the expression of genes controlling EMT in HCT116 and RKO cells transfected with siNC and siPRR14. (C) Western blot detection of the PRR14 and EMT indicators expression in xenograft tumor cells. (D) Statistics comparison of PRR14 and EMT indicators protein level in the xenografts. (E) Representative immunofluorescence images of phalloidin staining of HCT116 and RKO cells transfected with siNC and siPRR14, and the calculated proportion of pseudopod-positive cells. scale bar, 50 μm. (**: P < 0.01).