Mod antibody and RNAi validations.

(A) Merged image depicting Mod protein localization using anti-Mod antibody (blue), together with the nucleolar marker Fibrillarin (Fib; green) and NB marker Miranda (Mir; red), in a Control NB (1407>yw). Individual channels are depicted in greyscale in (B-D). (E) Merged image of staining of modL8 NBs using the same antibodies and imaging settings as above with individual channels represented in (F-H). NBs in modL8 brains are negative for Mod signal and total signal is abrogated. Scale bars represent 5μm. (I-P) Larval brains immunostained with Mir (Red), Fib (Green), and Mod (Blue) in Control (I-L) or modRNAi (M-P) using identical antibodies and imaging settings. RNAi expression decreases Mod signal in NBs. Scale bars represent 10μm.

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