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MiR-32 activates PI3K-Akt pathway by targeting toPTENand enhances Runx2 expression and phosphorylation.

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posted on 20.03.2017, 18:03 by Jianghua Liu, Xinhua Xiao, Yingying Shen, Ling Chen, Canxin Xu, Heng Zhao, Ying Wu, Qinghai Zhang, Jing Zhong, Zhenwang Tang, Changhui Liu, Qiang Zhao, Yi Zheng, Renxian Cao, Xuyu Zu

A, The miR-32 mimics (pre-miR32),negative control of miR-32 mimics (pre-con), miR-32 inhibitors (anti-miR32), or negative control of miR-32 inhibitors (anti-con)were transfected into MVSMCs and the cells were lysed and analyzed by immunobloting with the indicated antibody. *P< 0.05, **P< 0.01, ***P< 0.001, compared to control groups. B, Effect of miR-32 on PTEN expression by luciferase reporter assay. MVSMCs were cotransfected with pre-miR32 (or anti-miR32) and PTEN 3’UTR wild-type vectors (PTEN 3’UTR-WT) or PTEN 3’UTR mutant vectors (PTEN 3’UTR-MUT). Luciferase activity was normalized by the ratio of firefly and renilla luciferase signals. C, PI3K signaling is involved in miR-32 induced Runx2 expression and phosphorylation. MVSMCs were transfected with indicated concentration of miR-32 mimics or pre-con, with or without LY294002 and total protein extracts were analyzed by Western blot.Data of panel Aare analyzed with ANOVA with Bonferroni correction, and student t test is used for data anaysis of panel B&C. *P< 0.05,**P< 0.01.

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