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Methylation patterns of CRH promoter copies marked by histone-3, lysine-4 trimethylation (H3K4me3) and histone-4 acetylation (acH4).

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posted on 02.02.2017 by Xin Pan, Maria Bowman, Rodney J. Scott, John Fitter, Roger Smith, Tamas Zakar

(A) H3K4me3 and (C) acH4 at the CRH proximal promoter were measured by ChIP. Significant treatment effects on modification levels are indicated by asterisks at each promoter region (Fig 1A; *, p < 0.05, ANOVA with repeated measures, N = 3 placentae). (B) and (D) Methylation patterns of promoter copies marked by H3K4me3 and acH4, respectively, were determined in 8-Br-cAMP-treated cultures. Placenta numbers are on the left; filled and open circles indicate methylated and unmethylated CpGs, respectively. The positions of CpG Sites are as shown in Fig 1A. Notably, none of the H3K4me3- or acH4-associated epialleles had methylation patterns compatible with Pol-II or pCREB binding.