Methylation patterns of CRH promoter copies marked by histone-3, lysine-4 trimethylation (H3K4me3) and histone-4 acetylation (acH4).
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(A) H3K4me3 and (C) acH4 at the CRH proximal promoter were measured by ChIP. Significant treatment effects on modification levels are indicated by asterisks at each promoter region (Fig 1A; *, p < 0.05, ANOVA with repeated measures, N = 3 placentae). (B) and (D) Methylation patterns of promoter copies marked by H3K4me3 and acH4, respectively, were determined in 8-Br-cAMP-treated cultures. Placenta numbers are on the left; filled and open circles indicate methylated and unmethylated CpGs, respectively. The positions of CpG Sites are as shown in Fig 1A. Notably, none of the H3K4me3- or acH4-associated epialleles had methylation patterns compatible with Pol-II or pCREB binding.