Fig 5.tif (791.93 kB)

Methylation patterns of CRH promoter copies bound by TATA-binding protein (TBP).

Download (791.93 kB)
posted on 02.02.2017 by Xin Pan, Maria Bowman, Rodney J. Scott, John Fitter, Roger Smith, Tamas Zakar

(A) Binding of TBP to the CRH proximal promoter was determined by ChIP. Significant treatment effects at each promoter region (shown in Fig 1A) are indicated by asterisks (*, P < 0.05, ANOVA with repeated measures, N = 3 placentae; n.d, undetectable ChIP signal). (B) and (C), Methylation patterns of promoter copies bound by TBP in vehicle treated and 8-Br-cAMP-treated cultures, respectively. Arrows (←) on the right indicate patterns compatible with Pol-II binding, while (x) denotes methylation pattern compatible with binding by pCREB as shown in Fig 6B and 6D, respectively. Placenta numbers are on the left; filled circles indicate methylated CpGs and open circles indicate unmethylated CpGs. The positions of CpG Sites are as shown in Fig 1A.