Mechanisms of endocytosis of 2S albumins in bmDC and their uptake in CHO and CHO-MR.

(A.) The effect of macropinocytosis inhibitors, DMA and (B.) rottlerin, and (C.) mannose competitive inhibitor, mannan, on the endocytosis of rBer e 1-A488, nBer e 1-A488 and rSFA8-A488 into bmDC was determined by flow cytometry. (D.) The flow cytometry histogram of uptake of A488-labelled proteins by CHO and CHO-MR cell line, as analysed using flow cytometry (E.) The graph showed differences in the uptake of fluorescent-labelled rBer e 1, nBer e 1 and rSFA8 by CHO and CHO-MR where data are presented as normalised median fluorescent intensity (MFI) of cells pulsed with labelled proteins to unpulsed cells and presented as mean ±SD. ** p<0.01, *** p<0.001, ****p<0.0001. Statistical differences are relative to the untreated control.