Fig 7.tif (1.15 MB)

MeV-2 and MeV3 are present in M. euphorbiae WU11 population and is vertically transmitted to progeny.

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posted on 2018-03-29, 17:35 authored by Marcella A. Teixeira, Noa Sela, Hagop S. Atamian, Ergude Bao, Ritu Chaudhary, Jacob MacWilliams, Jiangman He, Sophie Mantelin, Thomas Girke, Isgouhi Kaloshian

Aphid nucleic acids were used in RT-PCR for MeV-2 and MeV-3 detection. For evaluation of vertical transmission, first instar nymphs were collected while being laid from adult aphids, before touching tomato leaflets, using a brush and transferred to a naïve tomato plant. One week later, when nymphs had molted into adults, single aphids were processed for the presence of MeV-2 and MeV-3. Aphid ribosomal gene RpL27 was used as positive control. M = molecular weight marker.