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Mating type loci in H.pseudoalbidus

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posted on 2014-04-25, 09:25 authored by Dan MacLeanDan MacLean, Diane Saunders

Mating-type loci analysis. Upper portion: The MAT locus in the KW1 isolate was identified in the genome sequence. A DNA polymerase zeta catalytic subunit was identified by tblastn of the region between the SLA2 and MAT1-1-3 genes against the NCBI nr database. Lower portion: H.pseudoalbidus PCR amplification of H. pseudoalbidus mating loci. AT indicates samples taken from Ashwellthorpe lower wood, KW1 is an isolate from Kenninghall wood that was previously confirmed as displaying the MAT1-1 (MAT1-1-3) mating type. ITS (internal transcribed spacer) analysis was used to confirm infection by H.pseudoalbidus.

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