Materials for fluorescence labeling

Copyright information:

Taken from "Protein–protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy"

Nucleic Acids Research 2006;34(14):e102-e102.

Published online Jan 2006

PMCID:PMC1904107.

© 2006 The Author(s).

() The structure of a fluorescent puromycin derivative. A fluorophore (Cy5 or RhG) and iminobiotin were chemically conjugated to puromycin through a linker. () DNA construction for fluorescence labeling of proteins. Template DNA consists of SP6 promoter, Omega sequence and an open reading frame (ORF) with a T7·tag at the N-terminus and a polyhistidine tag at the C-terminus, followed by a XhoI restriction enzyme site.