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Download fileMacrophage phenotypes change over time during type II and type III infection.
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posted on 24.10.2019, 20:09 by Shraddha Tuladhar, Joshua A. Kochanowsky, Apoorva Bhaskara, Yarah Ghotmi, Sambamurthy Chandrasekaran, Anita A. KoshyAt 5, 10, and 21 dpi, immune cells were isolated from the spleen of either type II- or type III-infected mice and stained and analyzed as in Fig 3. A,B. Quantification of the frequency and number of M2 (A) or M1-like (B) macrophages at 5 dpi. C,D. Quantification of the frequency (C) and number (D) of splenic M2 macrophages over time. E,F. Quantification of the frequency (E) and number (F) of splenic M1-like macrophages over time. G,H. Quantification of the frequency (G) and number (H) of splenic Tregs over time. Bars, mean ± SEM. N = 5–7 mice/infected group. *p<0.05, **p<0.01, two-way ANOVA with Fisher’s protected LSD. Data representative of 2 independent experiments. ● = type II, ■ = type III.
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type III Toxoplasma gondii survival Toxoplasma gondiitype III-infected micetype II-infected miceCNS T cellssubacute CNSM 2s T effector cellsrop 16 IIIROP 16-associated M 2 responseROP 16 III5 dpi type III-infected mice21 days post infectionlack ROP 16T cellsIII Δ rop 16 parasitesIII Δ rop 16type II-like neuroinflammatory responsePECtype III-associated M 2 responseIRGpro-inflammatory macrophagesM 2sIFNpolymorphic effector proteintype III parasites needtype III strainCNS parasite burden