ppat.1007856.g005.tif (788.03 kB)
Macrophage phenotypes change over time during type II and type III infection.
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posted on 2019-10-24, 20:09 authored by Shraddha Tuladhar, Joshua A. Kochanowsky, Apoorva Bhaskara, Yarah Ghotmi, Sambamurthy Chandrasekaran, Anita A. KoshyAt 5, 10, and 21 dpi, immune cells were isolated from the spleen of either type II- or type III-infected mice and stained and analyzed as in Fig 3. A,B. Quantification of the frequency and number of M2 (A) or M1-like (B) macrophages at 5 dpi. C,D. Quantification of the frequency (C) and number (D) of splenic M2 macrophages over time. E,F. Quantification of the frequency (E) and number (F) of splenic M1-like macrophages over time. G,H. Quantification of the frequency (G) and number (H) of splenic Tregs over time. Bars, mean ± SEM. N = 5–7 mice/infected group. *p<0.05, **p<0.01, two-way ANOVA with Fisher’s protected LSD. Data representative of 2 independent experiments. ● = type II, ■ = type III.
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type III Toxoplasma gondii survival Toxoplasma gondiitype III-infected micetype II-infected miceCNS T cellssubacute CNSM 2s T effector cellsrop 16 IIIROP 16-associated M 2 responseROP 16 III5 dpi type III-infected mice21 days post infectionlack ROP 16T cellsIII Δ rop 16 parasitesIII Δ rop 16type II-like neuroinflammatory responsePECtype III-associated M 2 responseIRGpro-inflammatory macrophagesM 2sIFNpolymorphic effector proteintype III parasites needtype III strainCNS parasite burden