MPP2 expression levels influence mIPSC amplitudes.

<p><b>(a)</b> Example traces of mIPSC in untreated (black) and MPP2-overexpressing (green) neurons. Scale bar: 100 pA/5s. <b>(b)</b> Overexpression of MPP2 leads to an increase in average mIPSC amplitude (unpaired <i>t</i> test <i>p</i> = 0.0403). Error bars of the boxplots correspond to the 10th and 90th percentile; control: <i>n</i> = 27; overexpression: <i>n</i> = 27. <b>(c)</b> Cumulative distribution of individual mIPSC amplitudes show a right-shift (larger amplitudes) in MPP2 overexpressing neurons. Kolmogorov–Smirnov test <i>p</i> < 0.0001. <b>(d)</b> Example traces of mIPSC from neurons transduced with control shRNA (black) and MPP2 knockdown shRNA (grey). Scale bar: 100 pA/5s. <b>(e)</b> Knockdown of MPP2 leads to a decrease in average mIPSC amplitude (Mann–Whitney test <i>p</i> = 0.0277). Error bars of the boxplots correspond to the 10th and 90th percentile; scramble: <i>n</i> = 58; KD: <i>n</i> = 40. <b>(f)</b> Cumulative distribution of individual mIPSC amplitudes shows a left-shift (smaller amplitudes) in MPP2 knockdown neurons (Kolmogorov–Smirnov test <i>p</i> < 0.0001). Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3001503#pbio.3001503.s016" target="_blank">S2 Data</a>. mIPSC, miniature inhibitory postsynaptic current; MPP2, membrane protein palmitoylated 2.</p>