MPP2 expression levels influence mIPSC amplitudes.

(a) Example traces of mIPSC in untreated (black) and MPP2-overexpressing (green) neurons. Scale bar: 100 pA/5s. (b) Overexpression of MPP2 leads to an increase in average mIPSC amplitude (unpaired t test p = 0.0403). Error bars of the boxplots correspond to the 10th and 90th percentile; control: n = 27; overexpression: n = 27. (c) Cumulative distribution of individual mIPSC amplitudes show a right-shift (larger amplitudes) in MPP2 overexpressing neurons. Kolmogorov–Smirnov test p < 0.0001. (d) Example traces of mIPSC from neurons transduced with control shRNA (black) and MPP2 knockdown shRNA (grey). Scale bar: 100 pA/5s. (e) Knockdown of MPP2 leads to a decrease in average mIPSC amplitude (Mann–Whitney test p = 0.0277). Error bars of the boxplots correspond to the 10th and 90th percentile; scramble: n = 58; KD: n = 40. (f) Cumulative distribution of individual mIPSC amplitudes shows a left-shift (smaller amplitudes) in MPP2 knockdown neurons (Kolmogorov–Smirnov test p < 0.0001). Underlying data can be found in S2 Data. mIPSC, miniature inhibitory postsynaptic current; MPP2, membrane protein palmitoylated 2.