# MO and EO circadian clocks are required for synchronization to temperature cycles in constant light.

A) Representative median of the normalized activity of control flies in LD. The white rectangle delimits the 12h of light while the grey box delimits the12h of darkness. This example explains the calculation of the slope (theoretical, Slo_{the}, and experimental, Slo_{exp}). Slo_{the} is calculated from the median levels according to the formula (Act_{ZTmax}- Act_{ZTmin})/(t_{ZTmax}-t_{ZTmin}) explained in Materials and Methods. For example here, the median activity of the first maximum before light-off (ZT11.5) is 67.6. Hence, here Slo_{the} = (67.6–0)/(691–511) = 0.38. The calculation is made with the ZT in minutes. Slo_{exp} is the median of the individual calculated slopes. B) Values of the Slo_{the}, Slo_{exp} and the ratio Slo_{exp}/Slo_{the} of the indicated genotypes in LD and LLTC6. If fly activity is highly synchronized between individuals, the ratio of Slo_{exp}/Slo_{the} is close to 1 (i.e., most individuals behave similar to the median), while for desynchronized populations the Slo_{exp}/Slo_{the} ratio is smaller (i.e., many individuals deviate from the median). C) In addition, we calculated the percentage of flies for each genotype with a Slo_{exp} > ½ of the Slo_{the} where a high versus low percentage again indicates synchronized or desynchronized behavior, respectively. Indeed, > 80% of the control flies increase their locomotion with Slo_{exp} > ½ Slo_{the}, compared to only 50–60% of the *Mai179>cyc*^{DN} and *cry[19]>cyc*^{DN} flies (S1C Fig, blue bars percentage of flies with Slo_{exp} > ½ of Slo_{the} ‘anticipating’, orange bars percentage of flies with Slo_{exp} < ½ of Slo_{the} ‘non-anticipating’). Same flies as in Fig 1B–1E).

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