MO and EO circadian clocks are required for synchronization to temperature cycles in constant light.

A) Representative median of the normalized activity of control flies in LD. The white rectangle delimits the 12h of light while the grey box delimits the12h of darkness. This example explains the calculation of the slope (theoretical, Slo_the, and experimental, Slo_exp). Slo_the is calculated from the median levels according to the formula (Act_ZTmax- Act_ZTmin)/(t_ZTmax-t_ZTmin) explained in Materials and Methods. For example here, the median activity of the first maximum before light-off (ZT11.5) is 67.6. Hence, here Slo_the = (67.6–0)/(691–511) = 0.38. The calculation is made with the ZT in minutes. Slo_exp is the median of the individual calculated slopes. B) Values of the Slo_the, Slo_exp and the ratio Slo_exp/Slo_the of the indicated genotypes in LD and LLTC6. If fly activity is highly synchronized between individuals, the ratio of Slo_exp/Slo_the is close to 1 (i.e., most individuals behave similar to the median), while for desynchronized populations the Slo_exp/Slo_the ratio is smaller (i.e., many individuals deviate from the median). C) In addition, we calculated the percentage of flies for each genotype with a Slo_exp > ½ of the Slo_the where a high versus low percentage again indicates synchronized or desynchronized behavior, respectively. Indeed, > 80% of the control flies increase their locomotion with Slo_exp > ½ Slo_the, compared to only 50–60% of the Mai179>cyc^DN and cry[19]>cyc^DN flies (S1C Fig, blue bars percentage of flies with Slo_exp > ½ of Slo_the ‘anticipating’, orange bars percentage of flies with Slo_exp < ½ of Slo_the ‘non-anticipating’). Same flies as in Fig 1B–1E).

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