MO and EO circadian clocks are required for synchronization to temperature cycles in constant light.
A) Representative median of the normalized activity of control flies in LD. The white rectangle delimits the 12h of light while the grey box delimits the12h of darkness. This example explains the calculation of the slope (theoretical, Slothe, and experimental, Sloexp). Slothe is calculated from the median levels according to the formula (ActZTmax- ActZTmin)/(tZTmax-tZTmin) explained in Materials and Methods. For example here, the median activity of the first maximum before light-off (ZT11.5) is 67.6. Hence, here Slothe = (67.6–0)/(691–511) = 0.38. The calculation is made with the ZT in minutes. Sloexp is the median of the individual calculated slopes. B) Values of the Slothe, Sloexp and the ratio Sloexp/Slothe of the indicated genotypes in LD and LLTC6. If fly activity is highly synchronized between individuals, the ratio of Sloexp/Slothe is close to 1 (i.e., most individuals behave similar to the median), while for desynchronized populations the Sloexp/Slothe ratio is smaller (i.e., many individuals deviate from the median). C) In addition, we calculated the percentage of flies for each genotype with a Sloexp > ½ of the Slothe where a high versus low percentage again indicates synchronized or desynchronized behavior, respectively. Indeed, > 80% of the control flies increase their locomotion with Sloexp > ½ Slothe, compared to only 50–60% of the Mai179>cycDN and cry>cycDN flies (S1C Fig, blue bars percentage of flies with Sloexp > ½ of Slothe ‘anticipating’, orange bars percentage of flies with Sloexp < ½ of Slothe ‘non-anticipating’). Same flies as in Fig 1B–1E).