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MOESM6 of Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

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posted on 2016-06-10, 05:00 authored by Christopher Landowski, Eero Mustalahti, Ramon Wahl, Laurence Croute, Dhinakaran Sivasiddarthan, Ann Westerholm-Parvinen, Benjamin Sommer, Christian Ostermeier, Bernhard Helk, Juhani Saarinen, Markku Saloheimo
Additional file 6: Figure S5. Immunoblot detecting IFNα-2b production. A. 1 liter bioreactor cultures Triab116-118. The M669, M670, and M672 were grown in 40 g/L lactose, 20 g/L spent grain extract, 20 g/L whole spent grain, 5 g/L (NH4)2SO4, 5 g/L KH2PO4 at pH 4.5, 28 °C. The base level of the control M577 strain was 0.50 g/L. The supernatants were diluted so that 0.2 µl was loaded per well. IFNα-2b standards corresponding to 50, 100, 200 ng were loaded for quantification. B. 1 liter bioreactor cultures Triab119 and 121. The M673 and M674 strains were grown in same media. The supernatants were diluted so that 0.2 µl was loaded per well. The base level of the control M577 strain was 0.50 g/L. IFNα-2b standards corresponding to 50, 100, 200 ng were loaded for quantification.

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