MOESM5 of EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

Additional file 5: Figure S5. Role of RA and Efhc2 in pronephros segmentation. Wild-type embryos or efhc2-Mo morphants were treated with DMSO, RA and DEAB. WISH showing expression of slc12a1 (DE), slc12a3 (DL), stc1 (CS) and odf3 (MCC). efhc2-Mo morphants treated with RA show expansion of expression domain of DE marker slc12a1 and almost or complete loss of DL and CS markers slc12a3 and stc1 as compared with wild-type embryos treated with RA. The expression domain and the number of cells expressing MCC maker odf3 was partially rescued by RA treatment in the morphants. DEAB treated efhc2-Mo morphants show expansion of DE, DL, and CS as compared with wild-type embryos treated with DEAB. The expression domain of odf3 is reduced in DEAB treated WT embryos.