MOESM4 of miR-1/206 downregulates splicing factor Srsf9 to promote C2C12 differentiation
figureposted on 03.12.2019, 04:41 by Kristen Bjorkman, Massimo Buvoli, Emily Pugach, Michael Polmear, Leslie Leinwand
Additional file 4: Figure S4. Endogenous Srsf9 mRNA and protein decrease with increased miR-1/206 expression. (A) Srsf9 mRNA levels decrease in C2C12 myoblasts transfected with miR-1 or miR-206 overexpression plasmids. Expression was assessed by qPCR and 18S rRNA was the reference gene. Expression levels were normalized to control myoblast levels (indicated by the dashed line). * = p ≤ 0.05; ** = p ≤ 0.01 vs. Control myoblasts. N = 3 independent cultures for each condition. (B) Srsf9 protein levels decrease in differentiating C2C12. Srsf9 was detected by western blot with a Srsf9-specific antibody (top panel). Ponceau S staining indicates equal protein loading (bottom panel).