12896_2020_600_MOESM3_ESM.jpg (37.45 kB)
Download file

MOESM3 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Download (37.45 kB)
posted on 2020-01-21, 05:13 authored by Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira, Mari Sogayar
Additional file 3: Figure S3. Second step of rhRSPO1 purification by molecular exclusion chromatography. The sample containing rhRSPO1 from step 2 of the heparin affinity column was used in this purification process. The blue line represents the absorbance at the 280 nm UV wavelength and the brown dotted line represents the conductance of the sample. A- Chromatogram representing the purification process of rhRSPO1 using a molecular exclusion column. B- Western Blot of the purification fractions, detecting the release of the target protein into the eluate. Tris-HCl buffer was used as a negative control (C-Buffer), and the Cl.L1 original conditioned media (OCM) and Step2 of the heparin column purification (Step2 Hep) were used as references.


Fundação de Amparo à Pesquisa do Estado de São Paulo