13068_2017_790_MOESM2_ESM.tif (272.41 kB)
MOESM2 of Process relevant screening of cellulolytic organisms for consolidated bioprocessing
figureposted on 2017-04-24, 05:00 authored by Elena Antonov, Ivan Schlembach, Lars Regestein, Miriam Rosenbaum, Jochen Büchs
Additional file 2: Figure S2. Characteristic growth and enzyme production properties of T. terrestris. (A) Biological duplicates of oxygen transfer rate (OTR) and cumulative oxygen transfer. For clarity of the depicted values, only every third measuring point of OTR and every eighth measuring point of cumulative oxygen transfer over time is represented by a symbol. (B) pH value and protein content in the culture supernatant; (C) Residual cellulose concentration and standard filter paper activity; RAMOS flasks were cultivated for OTR assessment without interruption. For offline analysis in B and C shake flasks run in parallel, inoculated from the same master mix, were harvested. Error bars represent standard deviation of technical triplicates from pooled biological duplicates. Culture conditions: modified Pakula medium with 5 g L−1 glucose and 30 g L−1 α-cellulose, 250 mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 106 spores mL−1, and 37 °C.