MOESM2 of Gambogic acid induces autophagy and combines synergistically with chloroquine to suppress pancreatic cancer by increasing the accumulation of reactive oxygen species
figureposted on 05.01.2019, 05:00 by Hongcheng Wang, Zhi Zhao, Shizhou Lei, Shaoli Li, Zhen Xiang, Xiaoyu Wang, Xiuyan Huang, Guanggai Xia, Xinyu Huang
Additional file 2. Rapamycin reduced the cytotoxicity of GA in pancreatic cancer cells. PANC-1 and BxPC-3 cells were pretreated with either 200 nM or 500 nM rapamycin for 24 h, and then treated with 1 µM GA for another 24 h. Cell viability was detected by the MTT assay. Data are presented as mean ± SD (n = 3); ** indicates P < 0.01, *** indicates P < 0.001.