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MOESM2 of Elevated HDAC activity and altered histone phospho-acetylation confer acquired radio-resistant phenotype to breast cancer cells

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posted on 2020-01-04, 04:40 authored by Asmita Sharda, Mudasir Rashid, Sanket Shah, Ajit Sharma, Saurav Singh, Poonam Gera, Murali Chilkapati, Sanjay Gupta
Additional file 2. (a) Representative z-stack projection images for immunofluorescence analysis of P and RR depicting changes in organization of α-tubulin. Magnification – 40x, scale bar- 10 μm. (b) Representative z-stack projection images for immunofluorescence analysis of P and RR depicting change in cellular morphology by PKH staining. Magnification – 40x, scale bar- 10 μm. (c) Graph depicting comparison of nuclear area between P and RR. Area was quantified from n = 50 DAPI stained nuclei. (d) Real time PCR based analysis depicts alteration in expression of different HDAC genes. Expression normalized to MCF7-parental. Fold change 1 depicts levels of parental MCF7. Images were processed using LSM browser software. Parental MCF7 is denoted as “P” and radioresistant cell line is denoted as “RR”. Statistical analysis is done by student’s t-test. n = 3 for all experiments. *p < 0.05, **p < 0.01 and a.u.- arbitrary units. Error bars represent ± S.D. of 3 experiments.

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SERB, DST, Government of India

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