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Ly6C+high, but not Ly6C+low, monocytes and neutrophils were recruited into ventilator-injured lungs.

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posted on 2016-10-26, 17:36 authored by Chung-Sheng Shi, Tzu-Hsiung Huang, Chin-Kuo Lin, Jhy-Ming Li, Mei-Hsin Chen, Mei-Ling Tsai, Chih-Ching Chang

(A) Gating strategy for differentiating Ly6C monocyte subsets and neutrophils. (B) Low SSC and CD11b-positive population (G1) was gated for further analysis of Ly6C and F4/80 or VEGF expressions. High Ly6C and F4/80 positive cells (G2) represented Ly6C+high monocytes. Low Ly6C and F4/80 positive cells (G3) represented Ly6C+low monocytes. The high SSC and CD11b-positive population (G4) represented neutrophils. High SSC and CD11b-positive population (G4) was gated for further analysis of Ly6C and F4/80 expressions. Intermediate high Ly6C and F4/80 negative cells (G5) represented neutrophils. Further, low SSC and CD11b positive population (G1) was gated to analyze the intracellular expression of VEGF in Ly6C monocytes. High Ly6C and VEGF-positive (G6) cells represented the VEGF expressing-Ly6C+high monocytes. Low Ly6C and VEGF-positive (G7) cells represented the VEGF expressing-Ly6C+low monocytes. (C) Time course for the recruitment of Ly6C+high, Ly6C+low monocytes, and neutrophils in the two-hit model of VILI. (D) Time course for the recruitment of VEGF expressing SSClowCD11b+Ly6C+high or Ly6C+low cells in the two-hit model of VILI. (Parenthesis): % of SSClowCD11b+Ly6C+high cells positive for VEGF. Values represent the mean ± SD (n = 4–6). #p <0.05 and *p <0.01, compared with the control of each time point.

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