Loss of clbn leads to ISCs over-proliferation and mitochondrial fragmentation independent of ribosome-associated quality control pathways.

(A-F) The posterior midguts of 15-day-old female flies of control (esg-lacz) (A), clbn ^KO (B), over-expression of dNOT4 (C), dUPF1 (D), deRF1 (E) and dXRN1 (F) in ECs under clbn ^KO background were stained with anti-β-gal antibody (green), anti-Prospero antibody (red) and DAPI (blue). (G) Quantification of the number of progenitor cells in flies of control (n = 10), clbn ^KO (n = 10), over-expression of dNOT4 (n = 10), dUPF1 (n = 10), deRF1 (n = 10) and dXRN1 (n = 10) in ECs under clbn ^KO background. (H) Quantification of the number of Pros⁺ cells in flies of control (n = 10), clbn ^KO (n = 10), over-expression of dNOT4 (n = 10), dUPF1 (n = 10), deRF1 (n = 10) and dXRN1 (n = 10) in ECs under clbn ^KO background.

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