Loss of both EXC-7 and MEC-8 show an additive effect on shared targets.
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Synchronized L4 worms were subjected to RNAi on NGM plates seeded with dsRNA-expressing bacteria. GFP was used as a non-targeting control. The L4 progeny of these worms were then collected using a COPAS worm sorter, and their RNA isolated for use in these RT-PCR experiments.