Loricrin is an important ligand during S. aureus SSTI.
Wild-type FVB (WT) and Lor-/- mice were infected subcutaneously with 2x107 CFU S. aureus LAC::lux or LAC::lux ΔclfB and abscess lesion size and bacterial burden was measured. Representative lesions from the dorsal area of mice from each group are shown (A) and results are expressed as total lesion size (cm2) ± SEM (B). Bioluminescence imaging was carried out using a Photon Imager and representative in vivo bioluminescence images are shown (C). Results are expressed as mean total photon flux (photons per second per steradian) ± SEM (D). Bacterial burden in the skin was assessed by viable counting on day 6 (E) post-infection. Results are expressed as Log10 CFU/mg. n = 5 per group. Tissue sections from WT and Lor-/- infected mice 12 h post-infection were stained with haematoxylin and eosin (F). Representative sections from each group are shown. Tissue sections were scored (double blind) for histology score (G). Abscess area was computed using ImageJ software and the areas were scored accordingly (H). Results expressed as mean score ± SEM. n = 4 per group. Two-way ANOVA with Bonferroni post-test (B, D), one-way ANOVA with Tukey post-test (E) and Mann-Whitney U test (G, H) used to analyze differences between groups. * P < 0.05, ** P < 0.01.