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Long-term impact of Sox2 deletion in the CvP.

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posted on 15.10.2020, 17:32 authored by Makoto Ohmoto, Weiwei Lei, Junpei Yamashita, Junji Hirota, Peihua Jiang, Ichiro Matsumoto

A: Lineage tracing of Sox2-deleted stem/progenitor cells together with immunohistochemical staining of KCNQ1 in the CvP of Krt5CreERT2/+; Sox2flox/flox; Rosa26lsl-Tom/+ mice (n = 2) 3 months after tamoxifen injection. B: Immunohistochemical staining of Ki67 in the CvP of Krt5CreERT2/+; Sox2flox/flox (n = 1) and Krt5CreERT2/+; Sox2flox/flox; Rosa26lsl-Tom/+ mice (n = 2) 3 months after tamoxifen injection. C-E: Expression of Krt5 (C), Lgr5 (D), and Sprr2a2 (E) in CvP of Krt5CreERT2/+; Sox2flox/flox; Rosa26lsl-Tom/+ mice (n = 3) after 3 months of tamoxifen injection. Mice without tamoxifen injection (–Tam) were used as controls. Dotted lines show the boundary of epithelium and connective tissue. Scale bars, 100 μm (B, C) and 50 μm (A, D, E).

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