pone.0214404.g003.tif (654.38 kB)

Long-term brain accumulation of anti-MOG antibody.

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posted on 12.04.2019 by Ryosuke Nakano, Sayaka Takagi-Maeda, Yuji Ito, Satoshi Kishimoto, Tomoko Osato, Kaori Noguchi, Kana Kurihara-Suda, Nobuaki Takahashi

Pharmacokinetic evaluation of peripheral and brain antibody level following intravenous injection of the negative control and of anti-MOG antibody MOG01. Mice were intravenously injected with anti-MOG antibody and control anti-AVM antibody (5 mg kg-1, n = 3 for each time point). After the times indicated, mice were perfused with PBS and their brains were extracted. The brain weights were measured, after which they were homogenized and eluted in citric buffer for antibody recovery. Blood was collected prior to perfusion from the tail vein. (A) Antibody concentration in the blood and (B) in the brain of MOG01 (black square) and negative control (white circle). Data represent the mean ± SD. **P<0.01 and ***P<0.001.