Localization of mCherry tagged PBANKA_0524300 (IPIS2), PBANKA_0623100 (IPIS3), and PBANKA_1400700 in the liver stage of infection.
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posted on 2022-09-20, 17:42 authored by Julie-Anne Gabelich, Josephine Grützke, Florian Kirscht, Oliver Popp, Joachim M. Matz, Gunnar Dittmar, Melanie Rug, Alyssa Ingmundson(A) Hepatoma cells were infected with transgenic parasites expressing the indicated proteins fused to the fluorescent protein mCherry. After fixation at the time points indicated, the infected cells were labelled with an anti-RFP antibody to amplify the mCherry signal. Scale bars, 10 μm. (B) P. berghei expressing both GFP- and mCherry-tagged proteins following cross fertilization were imaged by confocal microscopy 48 hours after infection of hepatoma cells. Scale bars, 10 μm
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successful parasite infectionerythrocytic membrane compartmentscharacterize protein componentshost membrane remodelingdiv >< pdata describing proteinsrich protein familythree candidate ibis1conserved across multiple>- infected cells>- infected erythrocytesplasmodium berghei required across speciesinfected cellsplasmodium p berghei infected erythrocyteserythrocytes infectedprotein exportrich domainhost erythrocyteinfected hostsidentified proteinsexported proteinscontaining proteinsvivo therefore failstage growthptex (<particular failfacilitate interactionsefficient sequestrationefficient bloodeffectively sequestercomponent exp2circulating bloodbind cd36better understandalso report
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