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Life span of various strains containing spilt-chromosomes

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posted on 30.12.2011, 16:02 authored by Yeon-Hee Kim, Daisuke Ishikawa, Ho Phu Ha, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima

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Taken from "Chromosome XII context is important for rDNA function in yeast"

Nucleic Acids Research 2006;34(10):2914-2924.

Published online 31 May 2006

PMCID:PMC1474064.

© The Author 2006. Published by Oxford University Press. All rights reserved

() Average life spans for FY833 (WT), ID-1, ID-2 and ID-8 strains were 34.1, 31.9, 20.6 and 22.7 generations, respectively. () Average life spans for the mutants, ID-10, ID-11, ID-12 and ID-13 were 42.5, 41.2, 30.1 and 19.9 generations, respectively. () Detection of the ERCs. DNA isolated from various strains was subjected to electrophoresis (0.6% agarose gel for 30 h at 1 V/cm) followed by Southern hybridization using 5S rDNA as a probe. Thin arrows represent ERCs and thick arrow indicates hybridization signal of the genomic rDNA copies. M; Lambda DNA/HindIII marker.

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