pgen.1009816.s004.tif (1.55 MB)
Download fileLcd1/Ddc2 is not required for NuA4 recruitment or resection in asynchronous cells.
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posted on 2021-09-20, 17:41 authored by Salar Ahmad, Valérie Côté, Xue Cheng, Gaëlle Bourriquen, Vasileia Sapountzi, Mohammed Altaf, Jacques Côté(A-B) ChIP-qPCR showing that the lcd1Δ xrs2Δ background results in loss of RPA signal (A), and NuA4 (B) at the HO induced DSB in asynchronous cells. The single mutant lcd1Δ (loss of Mec1) does not affect resection but shows slightly less NuA4 signal compared to wild type close to the break. (C-D) HO cutting efficiency at MAT locus after 3 hours of galactose induction in the indicated strains.
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histone acetylation targetsrepair pathway choicealternative repair mechanismsdiv >< pbudding yeast rad9nej1 inhibits repairhomologous end joiningfavors end resectioncore nhej factorsalso implicates nua4nhej factors yku80homologous endjoining machineryend resectionnhej factorsnhej mutantsvitro strand breaksstrand annealingresults establishresection phenotypepreviously reportedmimicking mutantgene regulationdirectly implicatedcell cycleantagonistic relationshipalso detect53bp1 ortholog
