Larvae fed with E. coli HT115 that expressed dsHaPtpn6 and then injected dsHaPtpn6 resulted in early pupation.
A. Phenotype analysis after feeding dsHaPtpn6 for 8 d and 10 d. B. Statistical analysis of early pupation in A by Student’s t-test based on three repeats. Thirty larvae for each repeat. C. The pupation time from 6th-0 h to pupa 0 d. D. The average pupal weight from the third instar feeding (3F) larvae to pupae 1 day (P-1 d) by Student’s t-test based on three repeats. Thirty larvae for each repeat. E. Efficiency analysis of HaPtpn6 knockdown using qRT-PCR at the mRNA level. F. Morphology of midgut after feeding dsRNA for 8 d. MG: midgut. Bar, 0.5 cm. G and H. HE staining of midgut and fat body after feeding dsRNA for 8 d. LM: larval midgut; IM: imaginal midgut. Bars, 20 μm. *P < 0.05 and **P < 0.01 using two-tailed Student’s t-test. The bars indicate the mean ± SD.