Laminin enhances the growth of human neural stem cells in defined culture media-3
figureposted on 31.12.2011, 20:37 by Peter E Hall, Justin D Lathia, Maeve A Caldwell, Charles ffrench-Constant
N2 or 2% B27 supplement, together with 0–100% neurosphere-conditioned medium. Relative to no conditioned medium (0%), a significant increase in neurosphere formation was observed for 50% conditioned medium only in the presence of N2 supplement. In comparison, 50% conditioned medium significantly reduced neurosphere formation in the presence of B27 supplement. Notably, human neurosphere formation was significantly increased by 2% B27 supplement compared to 1% N2 supplement for all concentrations of conditioned medium, as determined by a one-way ANOVA with Tukey post-test (< 0.001, = 2). (B) Graph showing the result of the limiting dilution assay, with cells from dissociated human neurospheres being plated at 10–60 cells per well in 2% B27 supplement. The number of non-responding wells (. without a neurosphere present) was quantified as a fraction of the total number of wells 21 days later. Linear regression was used to produce to the line of best fit. Unless otherwise noted, statistics were determined by a one-way ANOVA with Tukey post-test. = 2 for all except for the limiting dilution assay, where = 3. *< 0.05.
Taken from "Laminin enhances the growth of human neural stem cells in defined culture media"
BMC Neuroscience 2008;9():71-71.
Published online 23 Jul 2008