Laminin enhances the growth of human neural stem cells in defined culture media-2
figureposted on 31.12.2011, 20:37 by Peter E Hall, Justin D Lathia, Maeve A Caldwell, Charles ffrench-Constant
Ell in the presence of medium supplemented with either an isotype control antibody or a β1 integrin stimulatory antibody (TS2/16; both at 10 μg/ml). Note that no alteration to the number of primary neurospheres which formed was observed. (B) Primary neurospheres were dissociated and re-plated in medium alone. Again, TS2/16 had no effect on secondary neurosphere formation. (C) As for (A), except that the cells were grown in 2 ng/ml EGF and FGF-2, rather than 20 ng/ml. (D) Secondary neurosphere formation from the primary neurospheres in (C). Statistics were determined by a one-way ANOVA with Tukey post-test. (A) = 5; (B) = 4; (C, D) = 2.
Taken from "Laminin enhances the growth of human neural stem cells in defined culture media"
BMC Neuroscience 2008;9():71-71.
Published online 23 Jul 2008