LPS induces ferroptosis of lung epithelial cells.

<p>(A) CCK-8 assay to detect the cell number of MLE-12 cells treated with different concentrations of LPS (0.5, 1, 2, 5μg/mL) for 24 hours. (B) CCK-8 assay to detect the cell number of MLE-12 cells treated with 1μg/mL LPS for 6, 12, 24, and 48 hours. (C and D) WB assay to detect the protein level of FBLN1 in MLE-12 cells treated with 1μg/mL LPS for 6, 12, 24, and 48 hours. (E) Relative cell viability of the control, LPS (1 μg/mL), LPS (1 μg/mL) + Ferrostatin-1 (1 μM), and LPS (1 μg/mL) + Erastin (3 μM) treatment groups was measured by CCK-8 assay. (F) MDA kit assay to detect the effect of LPS (1 μg/mL), LPS+Ferrostatin-1 (1μM) and LPS+Erastin (3μM) on MDA concentration in MLE-12 cells. (G) A GSH kit was used to detect the effect of LPS (1 μg/mL), LPS+Ferrostatin-1 (1μM) and LPS+Erastin (3μM) on GSH concentration in MLE-12 cells. (H) The Fe²⁺ assay kit was used to detect the effect of different concentrations of LPS (0.5 μg/mL, 1 μg/mL, 2 μg/mL, and 5 μg/mL) on Fe²⁺ concentration in MLE-12 cells. (I) Fe²⁺ kit was used to detect the effect of LPS (1 μg/mL), LPS+Ferrostatin-1 (1μM) and LPS+Erastin (3μM) on Fe²⁺ concentration in MLE-12 cells. CCK-8, Cell counting kit-8; LPS, lipopolysaccharide; WB, Western blot; MDA, malondialdehyde; GSH, glutathione; Fe²⁺, ferrous iron. (J) FerroOrange probes were used to detect the impact of LPS (1 μg/mL), LPS+Ferrostatin-1 (1 μM), and LPS+Erastin (3 μM) on Fe2+ levels in MLE-12 cells. *<i>p</i><0.05.</p>