LDS-751 stains human embryonic stem cell mitochondria based on membrane potential.
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posted on 2012-12-19, 00:52 authored by Andrew B. J. Prowse, Fenny Chong, David A. Elliott, Andrew G. Elefanty, Edouard G. Stanley, Peter P. Gray, Trent P. Munro, Geoffrey W. Osborne(a) LDS-751 is co-localised with GFP in the KMEL2 mitochondria reporter line and does not overlap with the nucleus. Fluorescence intensities for each fluorophore were measured along the 160 µm line shown in the overlay image and plotted as distance vs intensity. (b) Mitochondria specific staining is lost when treated with a mitochondrial membrane depolarising agent valinomycin. Line profile analysis demonstrates LDS-751 no longer localised to the mitochondria after blocking mitochondrial membrane potential. The line profile in the overlay image represents 140 µm.
