ppat.1007029.g001.tif (304.46 kB)

LC/MS analysis of WNVKUN-infected cells reveals a homeostatic change in phospholipid levels.

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posted on 30.04.2018, 17:41 by Susann Liebscher, Rebecca L. Ambrose, Turgut E. Aktepe, Andrea Mikulasova, Julia E. Prier, Leah K. Gillespie, Adam J. Lopez-Denman, Thusitha W. T. Rupasinghe, Dedreia Tull, Malcolm J. McConville, Jason M. Mackenzie

Vero cells were mock- or WNVKUN-infected and harvested at 24 h.p.i. Levels of phospholipids were quantitated by LC/MS and the fold change plotted on GraphPad Prism 6. The analysis was performed on triplicate samples from triplicate experiments. (A) Lyso-phosphatidylcholine (Lyso-PChol), (B) Phosphatidylcholine (PChol), (C) Phosphatidylethanolamine (PE), (D) Phosphatidylserine (PS), (E) Phosphatidylinositol (PI). (F) Summary of significant changes in individual phospholipid species, and (G) Summary of fold change per phospholipid class.