Knockdown of miR-582-5p promotes proliferation and invasion of PC-9 cell.
(A) MiR-582-5p inhibitors were transfected into PC-9 cells and the silencing effect was assessed by using qPCR assay. (B) Cell growth rate was measured with CCK-8 assay. (C) The protein level of E-cadherin and Vimentin was detected by Western blot assay after PC-9 cells were transfected with miR-582-5p inhibitors. (D) Relative expression of E-cadherin and Vimentin was analyzed by using Image J software and GraphPad Prism 5.0. (E) The effect on PC-9 cell invasion mediated by miR-582-5p inhibitors was evaluated by Transwell assay. (F) The number of invasive cells was quantified with Image-Pro Plus and GraphPad Prism 5.0. Data are presented as the mean ± SD (n = 3). *P<0.05, **P<0.01 and **P<0.001 versus the scramble group.