Knockdown of klf4 increases the production of ludp

⁺sm13⁺cells. (A) FISH for tegumental markers with EdU detection in control (RNAi) and klf4 (RNAi) worms at day seven (D7) following an EdU pulse. Arrows represent EdU⁺ tegumental cells. (B) Quantification of the percentage of tegumental cells that are EdU⁺ following a 7-day chase period. Control (RNAi) n=28, klf4 (RNAi) n=29. (C) Double FISH for sm13 and ludp with EdU detection in control (RNAi) and klf4 (RNAi) worms at D7 following an EdU pulse. Red arrows represent EdU⁺sm13⁺ cells, white arrows represent EdU⁺ludp⁺ cells and red arrows with white outline represent EdU⁺ludp⁺sm13 cells. (D) Quantification of percentage of ludp⁺sm13⁺ cells that are EdU⁺ following a 7-day chase period following klf4 RNAi. Control (RNAi) n=16, klf4 (RNAi) n=16. Scale bar, 10 µm. Data are presented as mean ± SD. An unpaired t-test and a Welch’s t-test were performed in Panel B and D, respectively.