Fig 5.tif (344.23 kB)

Knockdown of endogenous psyllid mRNAs by dsRNA fed M. paniculata leaves after 11 days.

Download (344.23 kB)
figure
posted on 10.03.2017 by Diogo Manzano Galdeano, Michèle Claire Breton, João Roberto Spotti Lopes, Bryce W. Falk, Marcos Antonio Machado

M. paniculata leaflets were soaked in dsRNA solutions at a concentration of 500 ng.μL-1, and total RNAs were isolated from live third-instar nymphs after 11 days of feeding assays. GFP dsRNA-fed D. citri served as controls for each experiment. Expression levels of cathepsin D (A), chitin synthase (B) and inhibitor of apoptosis (C). Significant differences in relative expression were evaluated between test dsRNAs and GFP dsRNA at the same concentrations. Double asterisks indicate P<0.01. CD: cathepsin D; CS: chitin synthase; IA: inhibitor of apoptosis.

History

Licence

Exports