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Knockdown of endogenous psyllid mRNAs by dsRNA fed <i>M</i>. <i>paniculata</i> leaves after 11 days.

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posted on 2017-03-10, 18:28 authored by Diogo Manzano Galdeano, Michèle Claire Breton, João Roberto Spotti Lopes, Bryce W. Falk, Marcos Antonio Machado
<p><i>M</i>. <i>paniculata</i> leaflets were soaked in dsRNA solutions at a concentration of 500 ng.μL<sup>-1</sup>, and total RNAs were isolated from live third-instar nymphs after 11 days of feeding assays. GFP dsRNA-fed <i>D</i>. <i>citri</i> served as controls for each experiment. Expression levels of <i>cathepsin D</i> (A), <i>chitin synthase</i> (B) and <i>inhibitor of apoptosis</i> (C). Significant differences in relative expression were evaluated between test dsRNAs and GFP dsRNA at the same concentrations. Double asterisks indicate P<0.01. CD: <i>cathepsin D</i>; CS: <i>chitin synthase</i>; IA: <i>inhibitor of apoptosis</i>.</p>

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