JMY is not required for p53 stabilization, nuclear accumulation, phosphorylation, or acetylation.

(A) HAP1 and JMY^KO cells were treated with DMSO or 5μM etoposide for 6h before immunoblotting with antibodies to p53, p53^phospho-S15, p53^acetyl-K382, p53^phospho-S46, GAPDH, and tubulin. (B) HAP1 and JMY^KO cells were treated with DMSO or etoposide for 6h before being fixed and stained with a p53 antibody (green) and DAPI (DNA; blue). Scale bar, 30μm. (C) Cellular p53 fluorescence intensities were measured in ImageJ and the total p53 intensity was normalized to the HAP1 DMSO sample. Each bar represents the mean ±SD from 3 experiments (n = 196–343 cells per timepoint). AU = Arbitrary Units.

(TIF)