JMY is not required for p53 stabilization, nuclear accumulation, phosphorylation, or acetylation.
(A) HAP1 and JMYKO cells were treated with DMSO or 5μM etoposide for 6h before immunoblotting with antibodies to p53, p53phospho-S15, p53acetyl-K382, p53phospho-S46, GAPDH, and tubulin. (B) HAP1 and JMYKO cells were treated with DMSO or etoposide for 6h before being fixed and stained with a p53 antibody (green) and DAPI (DNA; blue). Scale bar, 30μm. (C) Cellular p53 fluorescence intensities were measured in ImageJ and the total p53 intensity was normalized to the HAP1 DMSO sample. Each bar represents the mean ±SD from 3 experiments (n = 196–343 cells per timepoint). AU = Arbitrary Units.