Isolation and identification of alginate-degrading bacteria.

The fermentation products of sample BSF were used in the isolation of alginate-degrading bacteria. Seaweed alginate-degrading bacteria A3 was isolated from the plates of VI plus seaweed alginate. Bacterial alginate-degrading bacteria P9 was isolated from the plates of VI plus P. aeruginosa alginate. The degradation function of A3 and P9 on alginates was detected by using TLC (A). VIA, culture media of VI plus seaweed alginate; VIP, culture media of VI plus P.aeruginosa alginate; No bacteria were represent in the control; A3 and P9, alginate-degrading bacterial strains A3 and P9 were inoculated. The 16S rRNA genes and alginate lyases genes of the bacterial strains A3 and P9 were identified by using PCR and sequencing. The similarity of the 16S rRNA gene (B) and alginate lyase gene (C) between A3 to P9 was analyzed by using the DNAStar software.