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Is a unique RNA cleaved by Ire1*

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posted on 2011-12-30, 14:43 authored by Maho Niwa, Christopher K Patil, Joe DeRisi, Peter Walter

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Taken from "Genome-scale approaches for discovering novel nonconventional splicing substrates of the Ire1 nuclease"

Genome Biology 2004;6(1):R3-R3.

Published online 22 Dec 2004

PMCID:PMC549064.

Copyright © 2004 Niwa et al; licensee BioMed Central Ltd.

Scatter plot of Cy3 and Cy5 signal intensities following hybridization to a yeast ORF microarray with both Cy3- and Cy5-labeled probes, prepared from the cleaved RNA fragments generated from either Ire1* or mock treatment, respectively, as described in Figure 1. Each point on the plot represents a single yeast ORF. Points below the diagonal represent ORFs that hybridize predominantly to RNAs represented in the Cy3 probe. The position of the spot displaying the brightest Cy3 signals corresponds to . Histogram representation of the logCy3/Cy5 ratio. Inset, is the only gene with a logCy3/Cy5 ratio near 2.3. All data displayed in Figure 3 are provided in Additional data file 1.

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