Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity-4

Ks domains 3 and 4, and the C-terminal HA tag. (B-F) H1299 cells engineered to express the above mutants (in two or three independent clones) were treated overnight (14 h) with 30 μg/ml FAC and lysates were subjected to Western blotting with HA (top) and β-actin (bottom) antibodies. The different migration of wild type IRP2 and the ΔC60 and ΔC168 deletion mutants is illustrated in (B).

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Taken from "Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity"

http://www.biomedcentral.com/1471-2199/9/15

BMC Molecular Biology 2008;9():15-15.

Published online 28 Jan 2008

PMCID:PMC2267205.