IpgB1 contributes to S. flexneri pathogenesis.

(A) Representative images of animals infected with wild type or ipgB1 S.flexneri 24 hr postinfection. (B and C) Histopathology scores for blood in stool (B) or diarrhea (C) of animals at 24 hr postinfection with wild type or ipgB1 strains. Each dot represents one animal and grey bars show average of six biological replicates. Error bars indicate standard deviation. Unpaired two-tailed t-test; *, p<0.05 (D) Representative images of hematoxylin- and eosin-stained colon sections from animals infected with wild type or ipgB1 at 24 hr postinfection. Black lines delineate the colon. Blue dashed lines indicate areas of intact epithelium and yellow dashed lines indicate areas with epithelial fenestration. Scale bar, 200 μm. (E) Graph depicting the percentage of epithelial fenestration along entire colon. Each dot represents one animal and grey bars show average of six biological replicates. Error bars indicate standard deviation. Unpaired two-tailed t-test; **, p<0.005.